rabbit poly clonal antibody against tpa  (Innovative Research Inc)

Journal: Blood

Article Title: Venous stasis-induced fibrinolysis prevents thrombosis in mice: role of α2-antiplasmin

doi: 10.1182/blood.2019000049

Figure Lengend Snippet: Expression of fibrinolytic system components in 24 h and 7 days old venous thrombi. Panels A-F show the expression (red) of (A-B) tPA, (C-D) uPA, (E-F) PAI-1, (G-H) plasminogen (Pg), (I-J) α2AP, and (K-L) their quantification in 8-µm cryosections of 24 hours and 7 days thrombi as labeled. Red color (Alexa Fluor 555) represents each component as labeled and blue color shows 4′,6-diamidino-2-phenylindole–stained nuclei. For comparison, IVC from sham mice were immunostained and quantified for each antibody under the same color range in the histogram mode of Image Pro-Plus software. The total immunostained area (arbitrary units; AU/mm2) for each protein was measured in 500 μm (original magnification ×4) images of an IVC sample. (N = 4-5 per group, mean ± SEM). *P < .05, **P < .01, ***P < .001.

Article Snippet: The primary antibodies include rabbit anti-mouse against tPA (ASMTPA-GF; Molecular Innovations, Novi, MI), uPA (ASMUPA-GF-HT; Molecular Innovations, Novi, MI), PAI-1 (IASMPAI-GF; Innovative Research, Novi, MI), plasminogen (Abcam; Cambridge, MA); goat anti-mouse α2AP (AF1239; R&D Systems, Minneapolis, MN); rat anti-mouse Ly6G (clone1A8, #127602; BioLegend, San Diego, CA), and Ly6B.2 (#771G; Bio-Rad Laboratories, Hercules, CA).

Techniques: Expressing, Labeling, Staining, Software